Regulatory

Part:BBa_J85702:Design

Designed by: Ron Weiss   Group: Weiss Lab   (2011-09-06)


Hef1a-LacO1Oid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 431
    Illegal PstI site found at 936
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal NheI site found at 307
    Illegal PstI site found at 431
    Illegal PstI site found at 936
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal BglII site found at 685
    Illegal BamHI site found at 270
    Illegal XhoI site found at 1084
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 431
    Illegal PstI site found at 936
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 431
    Illegal PstI site found at 936
    Illegal NgoMIV site found at 819
    Illegal AgeI site found at 95
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Position of Lac operators


Source

Sairam Subramanian

References